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SDS-PAGE comparison of native and recombinant PMT protein samples. Shown is a silver-stained SDS–8% polyacrylamide gel. Lanes: M, molecular weight markers (positions indicated in kilodaltons at the left); 1, 1.0 μg of native PMT (concentration based on that provided by supplier [Sigma]); 2, 0.1 μg of highly purified, native PMT; 3, 0.1 μg of rPMT before removal of the His tag; 4, 0.1 μg of rPMT after removal of the His tag.
