TABLE 1.

Deletion mutational analysis of PMT activity

Toxin protein	PMT residues	Calculated mol wt	Activity		EC50 (fmol/oocyte)
			Vero cell assay	Oocyte assay
Native PMT	1–1285	146,391	Yes	Yes	5.2
rPMT	1–1285	146,673	Yesa	Yesa	3.6
ToxAN3	1–93	11,028	Noa	Noa
ToxAN1	1–293	34,576	NTb	NTb
ToxAN2	1–505	60,464	Noa	Yesa	140
ToxAN4	1–568	67,077	No	Yes	22
ToxAN5	1–450	53,329	No	Yes	NDc
ToxAN6	1–400	48,176	No	No
ToxAN7	1–300	36,438	No	No
ToxAC1	505–1285	92,370	No	No
ToxAC2	1059–1285	29,355	No	No

^aTested after removal of the His tag. 

^bNT, not tested (not stable to thrombin treatment). 

^cND, not determined (only weak responses observed with injection of up to 3 pmol/oocyte).