FIG. 2.

IL-8 secretion by INT407 cells treated with C. jejuni 81-176 cells and fractions. INT407 monolayers were exposed to either whole cells or various fractions of C. jejuni 81-176 cells for 24 h, and IL-8 levels were measured by ELISA. The various samples were live 81-176 cells, live 81-176 cells plus 100 μg of chloramphenicol (CM)/ml, formalin-killed 81-176 cells, 15 μl of filter-sterilized culture supernatants from 18-h biphasic cultures of 81-176 cells, total membrane fraction purified from 81-176 cells (10 μg of total protein), soluble fraction purified from 81-176 cells (10 μg of total protein), and a control of MEM alone. Values are means and standard deviations from two to six experiments.