TABLE 2.
IL-8 secretion by INT407 cells after 24 h of exposure to C. jejuni strains
| Strain | Genotype | Relevant features (reference) | IL-8 secretion
|
% of wild typeb
|
||
|---|---|---|---|---|---|---|
| IL-8 (pg/ml)a | % of wild type | Adherence | Invasion | |||
| 81-176 strains | ||||||
| 81-176 | Wild type | 652 ± 34 | 100 | 100 | 100 | |
| K2-32 | flaA | Mot− FlaA− Inv− (42) | 120 ± 48* | 18 | 1.5 | 0.5 |
| RY213 | cheY+ diploid | Mot+ Che+ Inv− (43) | 194 ± 24* | 30 | 7.3 | 4.8 |
| RY303 | pflA | Mot− Che+ Inv− (42) | 175 ± 52* | 27 | 43 | 1 |
| RY224 | pspA | Mot+ Che+ Inv+; nonpiliated (13) | 622 ± 67 | 95 | 103 | 103 |
| RY209 | cheY | Mot+ Che−; hyperinvasive (43) | 1,482 ± 78* | 227 | 305.2 | 284 |
| 81-176 | peb1A | Mot+ Che+ Inv− (33) | 120 ± 25* | 18 | 10–50 | 5.5 |
| 749 strains | ||||||
| 749 | Wild type | 189 ± 44 | 100 | 100 | 100 | |
| 749 (cheY) | cheY | Mot+ Che−; hyperinvasive | 1,148 ± 22* | 607 | 317 | 133 |
a
The IL-8 levels are means and standard deviations from three or four independent assays. *, P < 0.001 compared to the appropriate wild-type strain.
b
The percent adherence and invasion for the peb1A mutant are taken from Pei et al. (33), those for the pspA mutant are from Doig et al. (13), and those for all other mutants are from Yao et al. (42, 43); the actual adherence for wild-type 749 was 0.035% ± 0.007%, and the actual invasion for wild-type 749 was 0.10% ± 0.002%.