Fig. 4.

ATF4 silence can induce NP cell’s apoptosis under the nutritional deficiency condition may via promoting AKT phosphate

(A) The AKT and P-AKT expression level detected by western blot under the condition of nutritional deficiency and ATF4 silence (Group A, B, C denotes the 10%FBS, 1%FBS and 1%FBS with siATF4 group respectively) (B) The quantity analysis of expression level of cell apoptosis protein as showed in A. Data are mean ± s.d. The percentage calculation in each tissue were measured at least 1000 cells each sample. Statistical significance was determined by one-way ANOVA and Student’s t-test. **, ##, △△ P < .001