Table 1.

Methylation-sensitive high-resolution melting primers, designed for promoter regions, and their amplicon information

Gene name	Sequence (5′ → 3′)	Ta˚C	Accession number	Number of CpG-sites/amplicon length
DIO3	F: GAG GGT ATT GTA GTA AGG TGT ATT R: AAA ACC CAA CCC ACC AAA TTC	58	NC_000014.9	18/271 bp
THRB	F: GTG TTA TTA GTT TGA TTA TTT GTT R: CTA TTC CAC CAC TAT CCA C	54	NC_000003.12	11/128 bp

Ta, appropriate annealing temperature; CpG, cytosine–phosphate diester–guanine