Table 1.

Thermal denaturation (T_m) and relationship to K_dimer of various MPro constructs in the absence and presence of GC373.

T_m was determined by DSF at a concentration of 10 µM in buffer B. M/D denotes monomer/dimer. ^a estimated by SV-AUC. ^b monitored by SEC-MALS. ^c cited from references.6, 10 DSF scans are shown in Figure 2. Green and red lettering denote the increase and decrease, respectively, of T_m and ΔT_m, relative to the corresponding mature MPro construct.