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. 2022 Nov 2;22:510. doi: 10.1186/s12870-022-03891-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Subcellular location of SsGRF1 protein in Nicotiana benthamiana leaf epidermal cells. The SsGRF1-GFP or GFP was transiently co-expressed with the nuclear localization marker NLS-mKATE by Agrobacterium. Images of epidermal cells were captured using green fluorescence, mKATE fluorescence, chlorophyll fluorescence, visible light, and merged light. Confocal laser microscopy scanning was carried out 48 h after dark culture with a Zeiss LSM 800. Scale bars, 20 μm