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. 2022 Nov 2;22:510. doi: 10.1186/s12870-022-03891-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — The expression pattern of GRF genes in Saccharum hybrid YT55 and YT00-236 based on log2-transformed FPKM values (A) under low-nitrogen stress conditions and verification of GRF1 and GRF3 expressions in root and leaf by RT-qPCR (B, C, D, and E). Seedlings of TY55 and TY00-236 were subjected to a nitrogen treatment of 100 mM, and samples were taken at 0, 6, 12, 24, 48, and 72 h after the treatment. The expression at 0 h was set to 1.0. Values were mean ± SD of three replicates. Bars with different letters were significantly different at the p < 0.05 level