Table EDT2:
Parameters of CryoEM procedures.
| Omicron BA.2 spike 2130-1-0114-112 | Omicron BA.2 RBD 2130-1-0114-112* | ||
|---|---|---|---|
| Data Deposition | EMDB | EMD-28198 | EMD-28199 |
| PDB | -- | 8EKD | |
| Microscope setting | Microscope | Krios | Krios |
| Voltage (kV) | 300 | 300 | |
| Detector | K3 | K3 | |
| Mag | X130,000 | X130,000 | |
| Pixel size | 0.647 | 0.647 | |
| Exposure (e-/Å2) | 52.2 | 52.2 | |
| Defocus range (μm) | 0.8–1.8 | 0.8–1.8 | |
| Data | # Micrographs | 18789 | 18789 |
| # particles | 1290705 | -- | |
| # particle after 2D | 844554 | -- | |
| Final particles # | 291461 | 386950 | |
| Symmetry | C3 | C1 | |
| Resolution FSC=0.143 |
3.26 | 3.6 | |
| Model refinement and validation | Protein residues | 441 | |
| Map CC | 0.63 | ||
| RMSD | |||
| Bond lengths (A) | 0.004 | ||
| Bond angles | 0.738 | ||
| Ramachandran | |||
| Outliers (%) | 0 | ||
| Allowed (%) | 6.02 | ||
| Favored (%) | 93.98 | ||
| Poor rotamers (%) | 0.88 | ||
| MolProbity score | 2.17 | ||
| Clash score | 18.79 |
*
: Focus refinement