Fig. 6.

Claudin-1 and claudin-4 expression in the ileum and colon following intratracheal LPS administration. Percentage area of claudin-1 expression was unchanged in both the ileum (A) and colon (B) following LPS administration. Relative expression of claudin-1 in the colon was also unchanged at both 24 and 72-h following LPS-adminstration (B’). No significant difference in percentage area of claudin-4 expression was was found in the ileum (C), however a redction in claudin-4 expression was found in the colon at 72-h post LPS administration (D). Relative expression of claudin-4, as measured via western blot, was unchanged in the colon (D’). Representative images of claudin-4 staining in the colon from vehicle (E’) and LPS-treated mice (E’’). LPS treatment had no effect on transepithial resistance (TER) in the ileum (F). Data were analysed by unpaired t-test with Welch's correction. Data presented as mean ± SEM. *P < 0.05 significantly different to vehicle control, n = 3–6 for ileum data, n = 5–6 for colon data