TABLE 6.
Commonly used NGS-based techniques for epigenetic analysis.
| Technique | Application | References |
|---|---|---|
| WGBS (Whole genome bisulfite sequencing) | The whole genome with all cytosine contexts can be studied | Lister and Ecker (2009) |
| MeDIP-seq (Methylated DNA Immunoprecipitation) | The whole genome can be studied with a small amount of initial DNA | Mohn et al. (2009) |
| RRBS (Reduced Representation Bisulfite Sequencing) | Can be applied to large-size genomes without a reference genomes and all cytosine contexts can be studied | David et al. (2017) |
| MSAP-seq (Methylation Sensitive Amplification Polymorphism Sequencing) | Can be applied for large-size genomes without a reference genomes | Chwialkowska et al. (2017) |
| ChIP-seq (Chromatin Immunoprecipitation Sequencing) | Can assess histone modifications; provides a genome-wide map | Diaz et al. (2017) |
| MS-DArT (Methylation Sensitive Diversity Array Technology) | The whole genome can be studied without a reference genomes | Pereira et al. (2020a) |