Figure 1.

Identification of an ARG2 peptide that elicits CD8⁺ T-cell responses. (A, left) IFN-γ ELISPOT screening of responses to 15 different HLA-A2 predicted ARG2-derived peptides in five healthy HLA-A2⁺ donors. 3×10⁵ cells were plated per well. Control and peptide stimulations were done in triplicates. Peptide-specific IFN-γ secreting cells are given as the difference of spots between cells in the peptide-stimulated wells and control wells. (A, right) Representative examples of the ELISPOT responses (shown in A). (B, left) IFN-γ ELISPOT responses of three HDs to A2S05. 3×10⁵ cells were plated per well. Control and peptide stimulations were done in triplicates. Bars represent the mean IFN-γ spot count±SD. (B, right) Representative examples of the ELISPOT responses (shown in B). (C) Representative intracellular cytokine staining for IFN-γ and TNF-α secreting in samples from three HDs stimulated with control or A2S05 gating strategy is available in online supplemental figure S6. DP: TNF-α⁺ IFN-γ⁺. DP, double positive; HD, healthy donor; IFN-γ, interferon gamma; PBMC, peripheral blood mononuclear cell; TNF-α, tumor necrosis factor alpha.