Fig. 5. Inhibitory secretion of TGF-β1 from a coculture of NK cells and HCC cells by apigenin.
Several cytokines were analyzed in the supernatants of a coculture of NK cells and HCC cells with or without treatment with CoCl2 (250 μM) and apigenin (50 μM) using ELISA assay. (A) IFN-γ, (B) TGF-β1, (C) IL-18, (D) IL-21, and (E) IL-10 secreted from a coculture of NK cells and HCC cells. The data are presented as means ± SD from three independent experiments. *p < 0.05 vs non-treated, **p < 0.05 vs CoCl2.