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. 2022 Feb 11;32(4):437–446. doi: 10.4014/jmb.2112.12036

Fig. 2. Incariin hydrolysis of enzyme fractions from DEAE-cellulose column in TLC, and purified enzyme in SDS-PAGE and HPLC.

Fig. 2

(A) Icariin hydrolysis of the enzyme fractions 6 to 176 from DEAE-cellulose column in TLC. 0.25% icariin was reacted at 40°C and pH 5.0 for 12 h. A, icariin; B, icariside II, l C, icaritin; 6 to 176, fraction number. Developing solvent, ethyl acetate: butanone: methanol: water = 8: 7: 1: 1 (v/v/v/v); rendering color in 280 nm ultraviolet. (B) Purified enzyme of 74 to 84 fractions in SDS-PAGE; E, purified enzyme; M, marker proteins including phosphatase b (97 kDa), bovine serum albumin (66 kDa), glutamic dehydrogenase (53 kDa), glyceralde-3-phosphate (36 kDa), trypsin inhibitor (24 kDa) and lysozyme (14.3 kDa). (C) Purified enzyme of 74 to 84 fractions in protein HPLC (73.2 kDa).