Fig. 3. Effects of pH and temperature on glycoside hydrolysis on icariin by purified enzyme.

(A) pH effect, 0.25% icariin was reacted in different pH by enzyme at 40°C for 12 h. (B) Temperature effect. 0.25% icariin was reacted in different temperature by enzyme at pH 5.0 for 12 h. A, icaritin; B, icariside II; C, icariin. Developing solvent, ethyl acetate: butanone: methanol: water = 8: 7: 1: 1 (v/v/v/v); rendering color in 280 nm ultraviolet.