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(A) Effects of different reaction time on 2.5% (w/v) icariin conversion at 40°C and pH 5.0 by crude enzyme in TLC. A, icaritin; B, icariside II; C, icariin; 3, 5,…to 24, reaction time (h). Developing solvent, ethyl acetate: butanone: methanol: water = 8: 7: 1: 1 (v/ v/v/v); rendering color in 280 nm ultraviolet. (B) Purity of icaritin product (0.04 mg/ml) in HPLC.
