Table 3.

Nile red-binding assay.

DMSO			Myricetin			p
Treatment (%)	Fluorescence (AU)	Relative fluorescence (%)	Treatment (µg/ml)	Fluorescence (AU)	Relative fluorescence (%)
0.1	22671 ± 441	100.0 ± 1.9	20	16325 ± 865	72.0 ± 3.8	< 0.001
0.2	24916 ± 1009	100.0 ± 4.0	40	16097 ± 991	64.6 ± 4.0	< 0.001
0.4	27214 ± 1390	100.0 ± 5.1	80	15157 ± 481	55.7 ± 1.8	< 0.001

C. albicans cells treated with myricetin or an equivalent amount of DMSO were incubated in PBS containing 0.25 mg/ml Nile red. The amount of Nile red binding to C. albicans cells was measured at 488 nm (bandwidth, 20 nm) and 580 nm (bandwidth, 20 nm), as the excitation and emission wavelengths, respectively, using a spectrofluorometer. The data represent the means ± standard deviations obtained from one of three independent experiments. AU: arbitrary units.