Fig. 3. Optimal conditions for polyhydroxybutyrate (PHB) degradation concerning temperature, NaCl concentration, the effect of the additional carbon and nitrogen sources.

(A) Microbulbifer sp. SOL03 cells were cultured at various temperatures ranging from 10°C to 42°C. (B) Microbulbifer sp. SOL03 cells were cultured with the addition of various concentrations of NaCl ranging from 0% to 5%. (C) Various carbon sources were added, including galactose (Gal), sucrose (Suc), glycerol (Gly), fructose (Frc), xylose (Xyl), lactose (Lac), and glucose (Glc). (D) Nitrogen sources were added, including ammonium sulfate [(NH₄)₂SO₄], ammonium nitrate [(NH₄)NO₃], ammonium dihydrogen phosphate [(NH₄)H₂PO₄], and ammonium chloride [(NH₄)Cl].