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. 2022 Jul 1;32(7):918–926. doi: 10.4014/jmb.2205.05012

Fig. 4. Cedrol induces apoptosis in A549 cells.

Fig. 4

(A) After treatment with indicated doses of cedrol for 48 h, cells were collected and stained with annexin V and 7-AAD. Induction of apoptosis by cedrol treatment was measured using a Muse Annexin V and Dead Cell Assay Kit with a Muse Cell Analyzer. (B) Percentages of early and late apoptotic cells were then calculated for each dose of cedrol. Data are presented as mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 vs. each control. (C) Effects of cedrol on the expression of apoptosis-related proteins were confirmed by western blot analysis after treatment with indicated doses of cedrol for 48 h. Actin was used as an internal control.