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. 2022 May 11;32(7):938–948. doi: 10.4014/jmb.2112.12044

Fig. 4. CircCOL1A2 regulates USP10 via competitively binding to miR-1286.

Fig. 4

(A) UALCAN (http://ualcan.path.uab.edu/) and starBase were utilized to predict the target mRNAs of miR-1286. (B) The expressions of PNPLA6, USP10 and ACTN4 were detected by qPCR in AGS cells after the knockdown of circCOL1A2. (C) USP10 expression in AGS, MKN-45 and GES-1 cells was detected by qPCR. (D-E) The binding sites between USP10 3’UTR and miR-1286 were shown. The interaction between USP10 and miR-1286 in GC cells was proved by RNA pull-down and luciferase reporter assays. (F) Western blot and qPCR were implemented to detect USP10 mRNA and protein levels in GC cells transfected with sh-NC, sh-circCOL1A2-1, sh-circCOL1A2-1+inhibitor NC or sh-circCOL1A2-1+miR-1286 inhibitor. One-way ANOVA and two-way ANOVA were used for comparison detection. **p < 0.01.