Skip to main content
. 2022 May 11;32(7):938–948. doi: 10.4014/jmb.2112.12044

Fig. 5. USP10 downregulates RFC2 ubiquitination level in GC cells.

Fig. 5

(A) STRING database (https://string-db.org/) and UALCAN were used to predict the proteins interacting with USP10 and RFC2 expression in GC. (B) Western blot and qPCR were used to evaluate RFC2 level after the ablation of USP10. (C) IF assay and bioinformatics proved the co-localization of USP10 and RFC2 in the nuclei of GC cells. (D) Co-IP in 293T cells assessed the interaction between RFC2 and USP10. (E) IP and western blot detected the ubiquitination level of RFC2 in 293T cells. (F) Western blot detected RFC2 level after CHX treatment and USP10 interference in AGS cells. One-way ANOVA was used for comparison detection.