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(A) The binding of wild-type FADD DED and Caspase-8 DEDs is shown using BIAcore biosensor analyte. (B-C) Interaction analyses of FADD DED and Caspase-8 DEDs (I128A) and Caspase-8 DEDs (R5E/I128A) are shown using BIAcore biosensor at 25°C. The FADD and Caspase-8 sensorgrams for 250, 1,000, 2,000, and 5,000 nM are shown.
