Table 2.
E9L-NVAR assay specificity to different viral/cellular DNAa (single assay) and sensitivity to vaccinia DNA (triplicate assays)
| Organism | Sample ID | DNA | 2 ng | 200 pg | 20 pg | 2 pg | 200 fg | 20 fg | 2 fg |
|---|---|---|---|---|---|---|---|---|---|
| Eurasian orthopoxvirusa | |||||||||
| Camelpox | E2379 | Partially pure | 21.26 | 24.60 | 29.01 | 34.18 | ND | ND | ND |
| Camelpox | v78-I-903 | Crude | 19.92 | 29.67 | 36.48 | ND | |||
| Cowpox | Brighton | Partially pure | 16.05 | 24.85 | 33.11 | ND | |||
| Ectromelia | Moscow | Partially pure | 17.10 | 20.35 | 25.54 | 28.57 | 31.68 | 37.43 | ND |
| Taterapox | Gerbilpox | Partially pure | 23.56 | 24.66 | 28.60 | 31.00 | ND | 39.25 | ND |
| Monkeypox | MPXV-ZAI-1996-016 | Partially pure | 15.29 | 19.94 | 22.49 | 27.10 | 30.50 | 35.70 | ND |
| Monkeypox | V70-266 Sierra Leone | Partially pure | 16.71 | 28.23 | 33.73 | ND | |||
| Monkeypox | MPXV-ZAI-1979-005 | Crude | 19.21 | 26.66 | 32.19 | ND | |||
| Vaccinia | Lister | Partially pure | 17.48 | 26.34 | 34.55 | ND | |||
| Vaccinia | Temple of Heaven | Partially pure | 17.13 | 28.32 | 35.86 | ND | |||
| Vaccinia | IHDW | Crude | 26.19 | 30.43 | 34.21 | 36.04 | ND | ND | ND |
| Vaccinia | Wyeth/Dryvax | Crude | 26.69 | 38.19 | ND | ND | |||
| Vaccinia | WYH pGS62-9-v1-1-1 | Partially pure | 19.33 | 23.60 | 36.71 | ND | |||
| Variola | SAF65-102 | Crude | ND | ND | ND | ND | |||
| Variola | SAF65-103 | Crude | ND | ND | ND | ND | |||
| Variola | 7124 | Crude | ND | ND | ND | ND | |||
| Variola | 7125 | Crude | ND | ND | ND | ND | |||
| Variola | Variolator 4 | Crude | ND | ND | ND | ND | |||
| Variola | Garcia | Crude | ND | ND | ND | ND | |||
| Variola | BSH | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | Butler | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | ETH72-17 | Crude | ND | ND | ND | ND | |||
| Variola | Harper | Crude | ND | ND | ND | ND | |||
| Variola | Harvey | Crude | ND | ND | ND | ND | |||
| Variola | Heidelberg | Crude | ND | ND | ND | ND | |||
| Variola | Higgins | Crude | ND | ND | ND | ND | |||
| Variola | Hinden | Crude | ND | ND | ND | ND | |||
| Variola | Horn | Crude | ND | ND | ND | ND | |||
| Variola | Iran 2602 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | Juba | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | K1629 | Crude | ND | ND | ND | ND | |||
| Variola | Kali Mathu | Crude | ND | ND | ND | ND | |||
| Variola | Hembula | Crude | ND | ND | ND | ND | |||
| Variola | Minnesota 124 | Crude | ND | ND | ND | ND | |||
| Variola | Lee | Crude | ND | ND | ND | ND | |||
| Variola | New Dehli | Crude | ND | ND | ND | ND | |||
| Variola | Nur Islam | Crude | ND | ND | ND | ND | |||
| Variola | Lahore | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | Rumbec | Crude | ND | ND | ND | ND | |||
| Variola | Shahzaman | Crude | ND | ND | ND | ND | |||
| Variola | Solaiman | Crude | ND | ND | ND | ND | |||
| Variola | Stillwell | Crude | ND | ND | ND | ND | |||
| Variola | V66-39 | Crude | ND | ND | ND | ND | |||
| Variola | V68-258 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | V68-59 | Crude | ND | ND | ND | ND | |||
| Variola | V70-222 | Crude | ND | ND | ND | ND | |||
| Variola | V70-228 | Crude | ND | ND | ND | ND | |||
| Variola | Congo | Crude | ND | ND | ND | ND | |||
| Variola | V72-119 | Crude | ND | ND | ND | ND | |||
| Variola | V72-143 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | Nepal 73 | Crude | ND | ND | ND | ND | |||
| Variola | V73-225 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | v74-227 Congo 9 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | V77-1252 | Crude | ND | ND | ND | ND | ND | ND | ND |
| Variola | v77-1605 | Crude | ND | ND | ND | ND | |||
| Variola | Yamada | Crude | ND | ND | ND | ND | |||
| Variola | Bombay | Crust | ND | ND | ND | ND | |||
| Variola | Hembula | Crust | ND | ND | ND | ND | ND | ND | ND |
| Variola | Mannan | Crust | ND | ND | ND | ND | ND | ND | ND |
| Variola | Kudano | Crust | ND | ND | ND | ND | |||
| Variola | Parvin | Crust | ND | ND | ND | ND | ND | ND | ND |
| Variola | Solaiman | Crust | ND | ND | ND | ND | ND | ND | ND |
| Variola | 7124 | Pure | ND | ND | ND | ND | ND | ND | ND |
| Variola | Variolator 4 | Pure | ND | ND | ND | ND | ND | ND | ND |
| Variola | BSH | Pure | ND | ND | ND | ND | ND | ND | ND |
| Variola | Horn | Pure | ND | ND | ND | ND | |||
| Variola | Nepal 73 | Pure | ND | ND | ND | ND | |||
| Variola | Somalia (SOM) | Pure | ND | ND | ND | ND | ND | ND | ND |
| Variola | V70-222 | Pure | ND | ND | ND | ND | ND | ND | ND |
| North American orthopoxvirusesa | |||||||||
| Raccoonpox | RCN | Partially pure | ND | ND | ND | ND | ND | ND | ND |
| Skunkpox | Skunkpox | Partially pure | ND | ND | ND | ND | |||
| Other rash illness or cellular DNAa | |||||||||
| Herpesvirus | HSV-1 HFEM | Pure | ND | ND | ND | ND | |||
| Herpesvirus | HSV-1 Justin | Pure | ND | ND | ND | ND | ND | ND | ND |
| Herpesvirus | HSV-2 patient 920 | Crust | ND | ND | ND | ND | ND | ND | ND |
| Herpesvirus | Varicella-OKA | Pure | ND | ND | ND | ND | ND | ND | ND |
| Herpesvirus | Varicella-WEB | Pure | ND | ND | ND | ND | |||
| Human | Human supT | Pure | ND | ND | ND | ND | |||
| Leporipox | Myxoma | Partially pure | ND | ND | ND | ND | |||
| Monkey | BSC-40 | Pure | ND | ND | ND | ND | |||
| Rickettsia | Rickettsia akari | Pure | ND | ND | ND | ND | ND | ND | ND |
| Rickettsia | Rickettsia conorii | Pure | ND | ND | ND | ND | |||
| Water | ND | ND | ND | ND | ND | ND | ND | ||
| Purified orthopoxvirus for determination of sensitivity | |||||||||
| Vaccinia | Wyeth/Dryvax | Pure | 16.07b | 19.47b | 23.41b | 27.53b | 31.56b | 35.69b | 39.68c |
a
The cycle when fluorescence crossed the threshold (Ct) for each positive sample is shown. ND, not detected.
b
Each sample was tested in triplicate, all three runs were positive, and the average Ct value is shown.
c
Each sample was tested in triplicate, one run was positive and two were negative, and the average Ct value is shown.