Fig. 4.

IgG1/IgG2a isotyping antibody responses in vaccinated mice. Serum pools from mice vaccinated with the 4pox DNA vaccine using Easy Vax™ (Easy Vax), scarified with live VACV (Scarified), vaccinated with the 4pox DNA vaccine using a gene gun (gene gun), or normal mouse serum (Neg Cont) were serially evaluated by VACV infected-cell-lysate ELISA. Serial dilutions of the pooled serum or antibody control mouse monoclonal antibodies (IgG1 MAb and IgG2a MAb) were tested for levels of IgG1, IgG2a, and total IgG. Specific O.D. for each dilution are represented as a stacked graph. ^*Below detection. Sera from mice vaccinated with the gene gun were obtained from a previously published study [6].