Table 1.

Immunogenicity and survival data for individual mice

Mouse ID	Antibody responsesa							Challengeb (day of death)
	ELISA	PRNT80	PRNT50	IFAT A27	IFAT B5	IFAT A33	FACS L1
4pox-Easy Vax DNA vaccine
268	1600	160	320	+	+	+	+	Survived
269	1600	80	640	+	+	+	−	Survived
270	6400	80	160	nd	nd	nd	nd	Survived
271	1600	80	160	+	+	+	−	Survived
272	1600	160	320	+	+	+	+	Survived
273	3200	160	320	+	+	+	+	Survived
274	3200	160	640	nd	+	+	+	Survived
275	1600	<20	20	hb	hb	hb	+	Survived
Negative control-Easy Vax DNA vaccine
276	<100	nd	nd	−	−	−	−	6
278	<100	nd	nd					6
279	<100	nd	nd					6
280	<100	nd	nd					6
281	<100	nd	nd					6
282	<100	nd	nd					7
283	<100	nd	nd					7
284	nd	<20	<20					8
285	nd	<20	<20					8
286	nd	<20	<20					8
287	nd	<20	<20					8
288	nd	<20	<20					7
289	nd	<20	<20					7
290	nd	<20	<20					8
Scarified VACV vaccine
291	6400	40	80					Survived
292	3200	80	320					Survived
293	6400	20	40					DUE
294	3200	40	160					Survived
295	6400	20	40					Survived
296	3200	40	80					Survived
297	6400	40	80					Survived
298	3200	40	40					Survived

PRNT, plaque reduction neutralization test where PRNT80 and PRNT50 is defined as the highest dilution reducing plaque number by 80% or 50%, respectively, relative to the number of plaques in the absense of test serum. IFAT, indirect immunofluorescence antibody test where cells were transfected with plasmid expressing A27, B5, or A33. FACS, fluorescently activated cell sorting where cells were transfected with pWRG/TPA-L1. ‘+’ Indicates positive signal relative to cells transfected with empty vector, − indicates negative signal. DUE, death unrelated to experiment. nd, not done; hb, high background.

^aELISA titer defined as lowest dilution aivina a specific O.D. areater than the mean specific O.D. of negative control serum (1:100) plus three standard deviations.

^bVACV strain IHD-J administered by intranasal route (2 × 10⁶ pfu).