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. 2019 Apr 11;95(1):41–45. doi: 10.1016/j.diagmicrobio.2019.03.015

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© 2019 Elsevier Inc. All rights reserved.

Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.

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Fig. 3 — Screening of 45 blood, plasma or serum samples from MXPV infected macaques and humans by real-time PCR and RPA assays. Linear regression analysis of real-time RT-PCR cycle threshold values (Ct) and RPA threshold time in minutes (TT) were determined. No correlation was found between TT and Ct values since the RPA is much faster than the real-time PCR. Diagnostic sensitivity of real-time PCR assay was 100%, while that of MPXV-RPA-assay was 95 %(43/45).