Table 2.

Oligonucleotide primers and hybridization probes used in multiplex real-time PCR.

Virus	Viral ORF	Oligonucleotide name	Oligonucleotide sequence
VARV	B12R	VARV_B12R_probe	FAM-5′-CTGTCGGAGCCACAGTTTCGAGACG-3′-BHQ1
		VARV_B12R_upper	5′-ATGTTCAAGCTGTTAATATCAATCTCG-3′
		VARV_B12R_lower	5′-TTTGCCACTGAACCATTCTATCAT-3′
MPXV	F3L	MPXV_F3L_probe	JOE-5′-TGTAGGCCGTGTATCAGCATCCATT-3′-BHQ1
		MPXV_F3L_upper	5′-CATCTATTATAGCATCAGCATCAGA-3′
		MPXV_F3L_lower	5′-GATACTCCTCCTCGTTGGTCTAC-3′
VZV	ORF38	VZV_ORF38_probe	TAMRA-5′-ACAATGAGTAGTGGCTTTATGGCGAG-3′-BHQ2
		VZV_ORF38_upper	5′-AAACCGCACATGATAACGC-3′
		VZV_ORF38_lower	5′-GATTAGGACCATCCCCCG-3′
IC	–	IC_probe	Cy5-5′-TTGCTTGTCGTGCTCGTATCGTCC-3′-BHQ3