Figure 4.

CQ induces accumulation of Atg8-family orthologs inside sEV of MDA-MB-231 cells. (A) Equal volume of secreted fraction collected from control and 10 µM CQ-treated MDA-MB-231 cells were treated with PBS, 50 µg/ml trypsin, or 50 µg/ml trypsin plus 1% Triton X-100 (TX-100). Positive controls for proteins inside sEV are TSG101 and SDCBP/syntenin-1. (B) Densitometry quantitation of trypsin-resistant SDCBP/syntenin-1, lipidated LC3B (LC3B-II), GABARAP, and GABARAPL2 levels normalized to TSG101. Significance was determined by paired ratio t-test (*: p < 0.05, n = 3). Error bars represent SD.