Figure 4.

Blocking DNAM-1 significantly inhibits the cytotoxic effects and cytokine secretion of γδ T cells. (a) Expanded γδ T cells were co-cultured with Calcein-AM-stained target cells for 4 hours at an E:T ratio of 20:1 in the presence of an isotype control or DNAM-1 blocking antibody. The % specific lysis was calculated as [(Experimental Release-Spontaneous Release)/(Maximum Release-Spontaneous Release)]×100. Experiments were carried out in duplicates using γδ T cells derived from 5 healthy donors. (b) The level of cytokine secretion from γδ T cells was analyzed after co-culture with two tumor PDG cells with an isotype control antibody or DNAM-1 blocking antibody. Cytokine secretion from only tumor cells was subtracted from the data and is also demonstrated separately. Experiments were carried out in duplicate using γδ T cells derived from five healthy donors and two PDG cells. Bars and error bars indicate mean and SD, respectively, paired t-test, *P < .05, **P < .005.