Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 9;18(11):2711–2730. doi: 10.1080/15548627.2022.2047344

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Informa UK Limited, trading as Taylor & Francis Group

PMC Copyright notice

Figure 5. — Rtn4 knockdown and vascular autophagic activation, as well as angiogenesis the ipsilateral thalamus after cortical infarction. (A) Immunoblotting shows the expression of S1PR2, RHOA and ROCK1 in vessels of the ipsilateral thalamus in the Scramble- and Rtn4-siRNA groups at 7 days after MCAO. (B-D) Quantitative analysis of S1PR2, RHOA and ROCK1 levels relative to TUBA. n = 4, data are expressed as median ± interquartile range. *P < 0.05, compared with the Scramble-siRNA group. (E) Immunoblotting shows the expression of MAP1LC3B-II, BECN1 and SQSTM1 in vessels of the ipsilateral thalamus in the Scramble- and Rtn4-siRNA groups. (F-H) Quantitative analysis of MAP1LC3B-II, BECN1 and SQSTM1 levels relative to GAPDH. n = 4, data are expressed as median ± interquartile range. *P < 0.05, compared with the Scramble-siRNA group. (I) Co-staining of BrdU (green) with LAMA1 (red) in the ipsilateral thalamus of the Scramble- and Rtn4-siRNA groups at 7 days after MCAO (arrows). Scale bar: 50 μm, 100 μm. (J and K) Quantitative analysis of BrdU⁺_LAMA1⁺ cells and vessel density. n = 6, data are expressed as median ± interquartile range. **P < 0.01, compared with the Scramble-siRNA group.