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. 2022 Mar 9;18(11):2711–2730. doi: 10.1080/15548627.2022.2047344

Figure 7.

Figure 7.

Treatment with 3-MA or spautin-1 enhanced angiogenesis in the ipsilateral thalamus after cortical infarction. (A) Immunoblotting shows the expression of MAP1LC3B-II in vessels of the ipsilateral thalamus in the vehicle and 3-MA groups at 7 days after MCAO. (B) Quantitative analysis of MAP1LC3B-II expression relative to GAPDH. n = 4, data are expressed as median ± interquartile range. *P < 0.05, compared with the vehicle group. (C) Co-staining for BrdU (green) and LAMA1 (red) within the ipsilateral thalamus of the vehicle and 3-MA groups at 7 days after MCAO (arrows). Scale bar: 50 μm, 100 μm. (D and E) Quantitative analysis of BrdU+_LAMA1+ cells and vessel density. n = 6, data are expressed as median ± interquartile range. *P < 0.05, compared with the vehicle group. (F) Immunoblotting shows MAP1LC3B-II expression in vessels of the ipsilateral thalamus in the vehicle and spautin-1 groups at 7 days after MCAO. (G) Quantitative analysis of MAP1LC3B-II expression relative to GAPDH. n = 4, data are expressed as median ± interquartile range. *P < 0.05, compared with the vehicle group. (H) Co-staining of BrdU (green) with LAMA1 (red) in the ipsilateral thalamus of the vehicle and spautin-1 groups at 7 days after MCAO (arrows). Scale bar: 50 μm, 100 μm. (I and J) Quantitative analysis of BrdU+_LAMA1+ cells and vessel density. n = 6, data are expressed as median ± interquartile range. *P < 0.05, compared with the vehicle group.