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. 2022 Mar 14;18(11):2697–2710. doi: 10.1080/15548627.2022.2047343

Figure 2.

Figure 2.

Prolonged myelin accumulation reduces autophagy levels in macrophages. (A-E) Immunofluorescence staining (A,D) and puncta-analysis (C,E) of bone marrow-derived macrophages (BMDMs) treated with myelin for a short (Mye-24) or prolonged (Mye-72) time. BODIPY (green), SQSTM1 (A, red), LC3 (D, red). Scale bar: overview 20 µm, inset 5 µm. (B) Quantification of number of BODIPY+-lipid droplets (LDs) in mye-24- and mye-72-BMDMs. Data originates from 3 independent experiments (50+ cells per condition). (F-H) Western blot analysis of LC3-II in untreated, mye-24- and mye-72-BMDMs stimulated with bafilomycin A1 (baf) 2 h prior to lysing (G) or non-stimulated (F). LC3-II level is calculated from 3 independent experiments and normalized to β-actin levels (loading control). All data are represented as mean ± SEM. *p < 0.05, **p < 0.01 and ****p < 0.0001.