Figure 6.

Trehalose reduces LD accumulation in late-stage microglia. (A,C) Immunofluorescence staining of primary mouse microglia treated with myelin for a short (Mye-24) or prolonged (Mye-72) time. BODIPY (green), LC3 (A, red), LAMP1 (C, magenta). Scale bar: overview 10 µm, inset 5 µm. (B,D) Average % of (B) LC3⁺ and (D) LAMP1⁺ lipid droplets (LDs) of the total amount of LDs. White arrows indicate colocalization. Quantification is calculated from 2 independent experiments (50+ cells per condition). (E) Representative images of Oil Red O staining of primary mouse microglia which were treated with myelin for a short (Mye-24) or prolonged (Mye-72) time and were additionally stimulated with trehalose (TOH) for the final 6 h of myelin-treatment. Scale bar: overview 50 µm, inset 20 µm. (n = 3 wells). All data are represented as mean ± SEM. *p < 0.05, ****p < 0.0001.