FIG. 3.

Binding of anti-blood group MAb to neutral glycosphingolipids. Neutral glycosphingolipids (100 μg) from phenotypes A (lane 2) and D (lane 3) pigs were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A) or incubated with the anti-Le^x (B), anti-H (C), anti-B (D), and anti-A (E) MAb as described in Materials and Methods. Lane 1 contains glycolipid standards Lc₂Cer (2), Gb₄Cer (4) and Gb₅Cer (5). The arrowheads indicate the positions of IGLad.