FIG. 4.

Binding of K88ad adhesin to intestinal neutral glycosphingolipids after β-galactosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with β-galactosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A), or incubated with biotinylated K88ad adhesin (B) or RCA₁₂₀ (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc₂Cer (2), Gb₄Cer (4) and Gb₅Cer (5). The arrowheads indicate the positions of IGLad.