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Binding of K88ad adhesin to purified neutral glycosphingolipids. Two micrograms of Lc3Cer (lane 1), Lc3Cer plus Lc4Cer (lane 2), nLc4Cer (lane 3), III4FucLc4Cer or Lea (lane 4), III3FucnLc4Cer or Lex (lane 5), V3FucnLc6Cer (lane 6), and VI2FucnLc6Cer (lane 7) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods.
