Figure 1.

ASIC1 expression in CALCA⁺and RET⁺ primary sensory neurons. (A) Representative confocal image showing the expression of ASIC1 (Acid-Sensing Ion Channel 1) in primary sensory neurons expressing RET (receptor tyrosine kinase RET) and CALCA (Calcitonin gene-related peptide). Τhe region within the rectangle is further highlighted on the right-side images. Double arrows point to cells which are double positive of ASIC1 and RET, and triple arrows point to cells expressing all the three genes. Scale bar = 50 μm. (B) Percentage of marker positive neurons expressing ASIC1; n = 3 DRGs from three donors. (C) Pie chart showing the distribution of ASIC1 positive neurons in the experiment #1; n = 1026 ASIC1⁺ neurons from three samples. (D) Histogram along with fitting curves of the neuronal size distribution (bin = 500 μm²) of ASIC1⁺ neurons and ASIC1/marker double positive neurons in human DRGs; Welch one-way ANOVA, W(DFn, DFd) = 2.112(2.000,3.874), P = 0.24, 584–1026 neurons from three samples. Detailed sample size can be found in Supplementary Table 1