Figure 3.

ASIC3 expression in CALCA⁺ and RET⁺ primary sensory neurons. (A) Representative confocal image showing the expression of ASIC3 (Acid-Sensing Ion Channel 3) in primary sensory neurons expressing RET (receptor tyrosine kinase RET) and CALCA (Calcitonin gene-related peptide). Τhe region within the rectangle is further highlighted on the right-side images. Double arrows point to cell expressing ASIC3 and RET, and triple arrows point to cells expressing all the three genes. Scale bar = 50 μm. (B) Percentage of marker positive neurons expressing ASIC3; n = 3 DRGs from three donors. (C) Pie chart showing the distribution of ASIC3 positive neurons in the experiment #3; n = 1088 ASIC3⁺ neurons from three samples. (D) Histogram along with fitting curves of the neuronal size distribution (bin = 500 μm²) of ASIC3⁺ neurons and ASIC3/marker double positive neurons in human DRGs; Welch one-way ANOVA, W(DFn, DFd) = 4.950(2.000,3.948), P = 0.08, 739–1088 neurons from three samples. Detailed sample size can be found in Supplementary Table 1