Fig. 5. ZIP reaction for backbone B. Schematic representation and UPLC traces of the CuAAC reaction of pre-ZIP 15 (50 μM in THF) after 22 h and 96 h, and after subsequent hydrolysis. Conditions: C18 column at 40 °C (254 nm) using water + 0.1% formic acid (A) and CH3CN + 0.1% formic acid (B); gradient of 0–3 minutes 5–95% B + 2 minutes 95% B.
