Fig. 6. ZIP reaction for backbone C. Schematic representation and UPLC traces for the CuAAC reaction of pre-ZIP 20 (50 μM in THF) after 3 h, 22 h and 9 days, and after subsequent hydrolysis. Conditions: C18 column at 40 °C (254 nm) using water + 0.1% formic acid (A) and CH3CN + 0.1% formic acid (B); gradient of 0–2 minutes 65–100% B + 1 minute 100% B for the CuAAC reaction (top three chromatograms), and gradient of 0–4 minutes 5–100% B + 1 minute 100% B for the hydrolysis (bottom chromatogram).
