Table 2. Mean structural parameters from SAXS data.
Batch SAXS and SEC–SAXS data were analysed individually, and the table reports the average value of each parameter with one standard deviation of their distribution given in parentheses. V P/m is the ratio of the Porod volume (V P) to the molecular mass (m). R g values for the consensus profiles are quoted with standard errors.
| Protein | Batch SAXS | SEC–SAXS | Consensus profile |
|---|---|---|---|
| RNaseA | |||
| R g, Guinier (Å) | 15.66 (0.26) | 15.08 (0.08) | 15.13 ± 0.02 |
| R g, P(r) (Å) | 15.55 (0.31) | 15.02 (0.08) | 15.04 ± 0.01 |
| d max (Å) | 50 (2) | 48 (1) | 49 |
| V P (Å3) | 16222 (682) | 15784 (577) | 17626 |
| V P/m | 1.18 | 1.15 | 1.29 |
| Lysozyme | |||
| R g, Guinier (Å) | 15.32 (0.81) | 15.05 (0.45) | 14.64 ± 0.05 |
| R g, P(r) (Å) | 15.33 (0.87) | 14.98 (0.38) | 14.46 ± 0.01 |
| d max (Å) | 49 (5) | 48 (3) | 48 |
| V P (Å3) | 19859 (4013) | 20324 (2553) | 18725 |
| V P/m | 1.38 | 1.42 | 1.31 |
| Xylanase | |||
| R g, Guinier (Å) | 17.18 (0.45) | 16.22 (0.22) | 16.05 ± 0.01 |
| R g, P(r) (Å) | 17.42 (0.60) | 16.17 (0.43) | 15.85 ± 0.01 |
| d max (Å) | 66 (7) | 58 (10) | 51 |
| V P (Å3) | 28601 (5143) | 26415 (3698) | 27151 |
| V P/m | 1.37 | 1.27 | 1.30 |
| Urate oxidase | |||
| R g, Guinier (Å) | 32.72 (0.53) | 31.96 (0.66) | 32.30 ± 0.06 |
| R g, P(r) (Å) | 32.18 (0.81) | 31.48 (0.51) | 31.63 ± 0.01 |
| d max (Å) | 104 (21) | 88 (4) | 92 |
| V P (Å3) | 217966 (30633) | 217723 (3777) | 219837 |
| V P/m | 1.60 | 1.59 | 1.61 |
| Xylose isomerase | |||
| R g, Guinier (Å) | 33.12 (0.31) | 33.15 (0.22) | 33.11 ± 0.05 |
| R g, P(r) (Å) | 32.96 (0.34) | 32.83 (0.08) | 32.93 ± 0.01 |
| d max (Å) | 98 (3) | 97 (2) | 101 |
| V P (Å3) | 234078 (5839) | 239819 (7908) | 243121 |
| V P/m | 1.35 | 1.39 | 1.41 |