Table 3. Structural parameters determined from SANS data.
Values for batch data are the average of individual analyses of multiple batch measurements, with one standard deviation given in parentheses. SEC–SANS values are from a single measurement with standard errors, while the consensus profile is from the optimal combination of batch and SEC–SANS data as described in the text, also with standard errors.
| Batch SANS H2O | SEC–SANS H2O | Consensus profile | Batch SANS D2O | SEC–SANS D2O | Consensus profile | |
|---|---|---|---|---|---|---|
| RNaseA | ||||||
| R g, Guinier (Å) | 14.93 (0.39) | 14.38 ± 0.38 | 14.74 ± 0.17 | 14.20 (0.52) | 13.64 ± 0.07 | 13.67 ± 0.08 |
| R g, P(r) (Å) | 15.00 (0.42) | 14.60 ± 0.16 | 14.50 ± 0.13 | 14.19 (0.46) | 13.78 ± 0.07 | 13.72 ± 0.05 |
| d max (Å) | 47 (3) | 43 | 41 | 46 (2) | 46 | 44 |
| Lysozyme | ||||||
| R g, Guinier (Å) | 15.11 (0.71) | 15.77 ± 0.41 | 14.28 ± 0.12 | 13.6 (0.33) | 12.16 ± 0.42 | 12.44 ± 0.52 |
| R g, P(r) (Å) | 14.99 (0.68) | 15.57 ± 0.27 | 14.66 ± 0.12 | 13.56 (0.28) | 12.81 ± 0.48 | 12.23 ± 0.10 |
| d max (Å) | 47 (1) | 48 | 48 | 46 (2) | 45 | 38 |
| Xylanase | ||||||
| R g, Guinier (Å) | 16.82 (0.39) | 15.87 ± 0.38 | 16.03 ± 0.30 | 15.86 (0.77) | 14.65 ± 0.24 | 14.87 ± 0.19 |
| R g, P(r) (Å) | 16.89 (0.37) | 15.38 ± 0.17 | 15.02 ± 0.19 | 15.69 (1.03) | 14.58 ± 0.07 | 14.43 ± 0.05 |
| d max (Å) | 55 (6) | 43 | 43 | 53 (5) | 46 | 44 |
| Urate oxidase | ||||||
| R g, Guinier (Å) | 31.66 (1.01) | 30.34 ± 2.43 | 31.57 ± 0.42 | 32.32 (1.90) | 31.50 ± 0.34 | 31.18 ± 0.12 |
| R g, P(r) (Å) | 32.25 (1.37) | 29.52 ± 0.39 | 31.67 ± 0.23 | 30.92 (0.44) | 30.03 ± 0.06 | 30.84 ± 0.04 |
| d max (Å) | 94 (4) | 83 | 91 | 91 (2) | 83 | 93 |
| Xylose isomerase | ||||||
| R g, Guinier (Å) | 32.00 (1.19) | 31.51 ± 1.58 | 32.61 ± 0.38 | 30.79 (0.69) | 30.59 ± 0.12 | 30.72 ± 0.17 |
| R g, P(r) (Å) | 32.21 (0.65) | 33.01 ± 0.49 | 32.30 ± 0.18 | 30.97 (0.68) | 30.58 ± 0.05 | 30.88 ± 0.08 |
| d max (Å) | 93 (6) | 95 | 97 | 93 (6) | 90 | 95 |