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(a) PDB entry 3now (light green) adopts a V-shaped conformation. The processed AlphaFold prediction (coloured by B factor; blue, 0 Å2; red, ≥100 Å2) has a significantly wider hinge angle, causing a severe clash of the N-terminal domain with a symmetry mate (green Cα trace). (b) Using two domains identified from the PAE matrix by phenix.process_predicted_model (purple, orange) yields a strong MR solution.
