FIG. 5.

Expression of Esp on the surface of E. faecalis and lack of cross-reactivity with GBS proteins Rib and C alpha. (A) Bacterial suspensions (1%) were incubated with dilutions of rabbit antiserum as indicated, and bound antibodies were detected after incubation with radiolabeled protein G. (B) Bacterial suspensions were incubated with antiserum (diluted 1:500), and bound antibodies were detected after incubation with radiolabeled protein G. The strains evaluated were E. faecalis MMH594 (this study), Rib-positive group B streptococcal strain BM110 (49), and C alpha-positive group B streptococcal strain A909 (33). The results are expressed as a percentage of the total radioactivity added. Values represent the mean of duplicate tests with similar results. Controls with preimmune rabbit serum were included in all experiments and gave completely negative results in all cases.