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. 2022 Oct 21;18(10):e1010623. doi: 10.1371/journal.pcbi.1010623

Fig 2. ISG induction after IFN stimulation.

Fig 2

A: Experimental set-up showing the graded expression of MxA and IFIT1 after IFN-α stimulation in a population of Huh7.5 cells. A threshold is defined to differentiate responder vs. non-responder cells in the population. B: Distributions represent the flow cytometry measurements of MxA expression at different time points after IFN stimulation (blue distribution). C: Distributions represent the flow cytometry measurements of IFIT1 expression at different time points after IFN stimulation (red distribution). In figures B and C, the vertical dashed lines represents a threshold value (defined as the mean plus two standard deviations) calculated form the unstimulated populations. This threshold value is used to differentiate responser vs non-responder cells in a cell population with a graded response. In the observed treatments, the mean fluorescence level shifts from 8x103 a.u. (arbitrary units of fluorescence) to 4.5x104 a.u. for MxA, and from 2x104 to 9x104 a.u. for IFIT1.