Table 1. Overview of studies and high-throughput techniques to describe endogenous and etoposide-induced TOP2-DNA interactions and DNA DSBs in human and mouse.
| Endogenous TOP2 functions | Etoposide-directed TOP2 functions | ||
| TOP2A binding | TOP2B binding | ||
| ChIP-seq | Reference | ChIP-seq | Reference |
| •Mouse MEFs | (21) | •Human MCF10A-AsiSI cells | (89) |
| •Mouse thymocytes | (90) | •Human glioblastoma cell lines, tumors | (151) |
| •Human RPE-1 cells | (92) | ||
| TOP2B binding | TOP2cc maps | ||
| ChIP-seq | TOP2Acc-mapping | ||
| •Mouse primary neurons | (97) | •Human K562 cells | (85) |
| •Human MCF7 cells | (87, 91) | CC-seq | |
| •Mouse liver | (86) | •Human RPE-1 cells | (93) |
| •Mouse MEFs | (21, 88) | DSB maps | |
| •Mouse thymocytes | (90) | DSB-seq, SSB-seq | |
| •Human MCF10A-AsiSI cells | (89) | •Human HCT116 cells | (95) |
| •Human glioblastoma cell lines, tumors | (151) | END-seq | (21, 88, 96) |
| •Mouse B cells, T cells, MEFs, primary neurons | |||
| ChIP-exo | |||
| •Mouse liver | (86) | •Human MCF7, Nalm6, HCT116 cells | |
| DSB maps | |||
| BLISS | BLISS, sBLISS | ||
| •Human MCF10A-AsiSI cells | (89) | •Human TK6, K562, CD34+ cells | (22) |
| DSBCapture | DSBCapture | ||
| •Human HeLa cells | (94) | •Human HeLa cells | (94) |
| TOP2B protein-protein interactions | |||
| BioID | |||
| •Human HeLa cells | (86) | ||