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Selection of T0 rice plants transformed with the construct for AtpD overexpression (AtpD-OE). Immunodetection of AtpD-Myc in leaf protein extracts, 15 µg of protein loaded for each sample. The copy number of the hygromycin phosphotransferase gene (hpt) detected by digital PCR was used to estimate the number of construct insertions. *Lines 2, 9, and 15 were selected for further experiments.
