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. 1999 Jan;67(1):206–212. doi: 10.1128/iai.67.1.206-212.1999

FIG. 6.

FIG. 6

Synergistic induction of luciferase by TNF-α or IL-1 in association with IFN-γ. RAW cells were transfected with a luciferase reporter gene driven by the pIRE. Cell extracts were prepared and assayed for luciferase activity as described earlier (19). The data are means ± the standard errors of the means of triplicate measurements. The various treatments applied (for 16 h) are indicated at the bottom. A minus or plus sign indicates the absence or presence, respectively, of the given agent during incubation. An anti-TNF-α antibody (2.5 μg/ml) and/or an anti-IL-1R antibody (2.5 μg/ml) were added to the culture 30 min prior to treatment with the indicated cytokines. IFN-γ (50 U/ml), IL-1 (25 ng/ml), and TNF-α (10 ng/ml) were used in the experiment.

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