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. 2022 Oct 19;15:1020803. doi: 10.3389/fnmol.2022.1020803

FIGURE 2.

FIGURE 2

Co-transduction of dual AAV-ie vectors in the normal utricle of neonatal mice. AAV-ie-CAG-EGFP and AAV-ie-CAG-mCherry were injected into the neonatal mice at postnatal day 1 (P1). The utricles were sampled at P15. (A–A”’) Low-magnification images. (B–C”’) Numerous hair cells (HCs) (B–B”’; arrowheads; representative images of the extrastriolar region) and supporting cells (SCs) (C–C”’; arrows; representative images of the striolar region) express both GFP and mCherry. Scale bars, 50 μm in A for (A–A”’); 20 μm in B for (B–C”’). (D,E) Comparative analysis of the co-transduction rates of HCs (D) and SCs (F) between normal neonatal and adult mice. The co-transduction rates of HCs in neonatal mice are significantly higher than in adult mice in striolar and extrastriolar regions, whereas the co-transduction rates of SCs are comparable between the groups. Data are mean ± SEM. P-values were calculated using Student’s t-test. “ns”, not significant. **P < 0.01.